human follicular thyroid cancer–derived cell line ftc-133  (Shilpa Medicare)

Journal: Molecular Therapy Oncolytics

Article Title: Gene Silencing of Transferrin-1 Receptor as a Potential Therapeutic Target for Human Follicular and Anaplastic Thyroid Cancer

doi: 10.1016/j.omto.2020.01.003

Figure Lengend Snippet: Effect of RNA Interference on the Percentage of Cell Viability for 8305C Cells

Article Snippet: Follicular (FTC-133) and anaplastic (8305C) human thyroid cancer cells lines were obtained from the Cell Bank Interlab Cell Line Collection (Genoa, Italy).

Techniques: Inhibition

Journal: Molecular Therapy Oncolytics

Article Title: Gene Silencing of Transferrin-1 Receptor as a Potential Therapeutic Target for Human Follicular and Anaplastic Thyroid Cancer

doi: 10.1016/j.omto.2020.01.003

Figure Lengend Snippet: Effect of siRNA-TfR1 Transfection on Growth Curve and on Morphological Changes in FTC-133 and 8305C Cell Line Cultures (A and C) FTC-133 (A) and 8305C (C) human thyroid cancer cell line proliferation rates through kinetics of cell growth curve analysis performed in 10 mM scrambled and siRNA-TfR1-transfected cells for 24, 48, and 72 h. Values represent the mean ± SD of five separate experiments performed in triplicate. *p < 005 versus scrambled cells used as a control. (B and D) Morphology evaluation of (B) FTC-133 and (D) 8305C cells. Morphological changes are apparent in FTC-133 and 8305C cells at 48 and 72 h. Magnification is equal in all images. Scale bars, 20 μm.

Article Snippet: Follicular (FTC-133) and anaplastic (8305C) human thyroid cancer cells lines were obtained from the Cell Bank Interlab Cell Line Collection (Genoa, Italy).

Techniques: Transfection, Control

Journal: Molecular Therapy Oncolytics

Article Title: Gene Silencing of Transferrin-1 Receptor as a Potential Therapeutic Target for Human Follicular and Anaplastic Thyroid Cancer

doi: 10.1016/j.omto.2020.01.003

Figure Lengend Snippet: Effect of siRNA on mRNA Expression for TfR1 through Semiquantitative RT-PCR Analysis on FTC-133 and 8305C Cell Line Cultures (A) Representative semiquantitative RT-PCR analysis for TfR1 mRNA expression in FTC-133 and 8305C human thyroid cancer cells lines scrambled (TfR1 + ) and 10 nM siRNA-TfR1 transfected for 48 h (TfR1 − ). (B) Densitometric analysis of RT-PCR products performed after normalization through using GAPDH as housekeeping gene. Results are expressed as the mean ± SD of the values of five separate experiments performed in triplicate. *p < 0.05 for differences versus controls.

Article Snippet: Follicular (FTC-133) and anaplastic (8305C) human thyroid cancer cells lines were obtained from the Cell Bank Interlab Cell Line Collection (Genoa, Italy).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Transfection

Journal: Molecular Therapy Oncolytics

Article Title: Gene Silencing of Transferrin-1 Receptor as a Potential Therapeutic Target for Human Follicular and Anaplastic Thyroid Cancer

doi: 10.1016/j.omto.2020.01.003

Figure Lengend Snippet: Effect of siRNA Gene Silencing on TfR1 Expression Levels through Immunofluorescence and Western Blotting Analysis on FTC-133 and 8305C Cell Line Cultures (A) Positive immunofluorescence microscopic analysis for TfR1 in scrambled (a) FTC-133 and (c) 8305C human thyroid cancer cells lines and 10 nM siRNA-TfR1-transfected cells for 48 h. (b) FTC-133 and (d) 8305C human thyroid cancer cell lines. Magnification is equal in all images. Scale bars, 40 μm. (B) Representative immunoblot through western blotting analysis for TfR1 expression levels in total cellular lysates from scrambled and 10 nM siRNA-TfR1-transfected cells for 48 h. (C and D) Densitometric analysis of TfR1 expression (performed after normalization with β-tubulin) in both cell lines. Results are expressed as the mean ± SD of the values of five separate experiments performed in triplicate. *p < 0.05 for differences versus controls.

Article Snippet: Follicular (FTC-133) and anaplastic (8305C) human thyroid cancer cells lines were obtained from the Cell Bank Interlab Cell Line Collection (Genoa, Italy).

Techniques: Expressing, Immunofluorescence, Western Blot, Transfection

Journal: Molecular Therapy Oncolytics

Article Title: Gene Silencing of Transferrin-1 Receptor as a Potential Therapeutic Target for Human Follicular and Anaplastic Thyroid Cancer

doi: 10.1016/j.omto.2020.01.003

Figure Lengend Snippet: Effect of siRNA Gene Silencing on ERK1/2 Pathway through Western Blotting Analysis on FTC-133 and 8305C Cell Line Cultures (A) Representative immunoblots of ERK1/2 expression levels in total cellular lysates from scrambled FTC-133 and 8305C human thyroid cancer cell lines and 10 nM siRNA-TfR1-transfected cells for 48 h. (B) Densitometric analysis of ERK1 and ERK2 expression levels in response to siRNA-TfR1 gene silencing in transfected FTC-133 and 8305 human thyroid cancer cell lines, when compared with scrambled ones, used as control (performed after normalization with β-tubulin). (C) Representative immunoblot of c-Myc expression levels in total cellular lysates from scrambled FTC-133 and 8305C human thyroid cancer cell lines and 10 nM siRNA-TfR1 transfected cells for 48 h. (D) Densitometric analysis of c-Myc (performed after normalization with β-tubulin) in response to siRNA-TfR1 transfection, when compared with scrambled ones, used as control. Results are expressed as the mean ± SD of the values of five separate experiments performed in triplicate. *p < 0.05 for differences versus controls.

Article Snippet: Follicular (FTC-133) and anaplastic (8305C) human thyroid cancer cells lines were obtained from the Cell Bank Interlab Cell Line Collection (Genoa, Italy).

Techniques: Western Blot, Expressing, Transfection, Control

Journal: Molecular Therapy Oncolytics

Article Title: Gene Silencing of Transferrin-1 Receptor as a Potential Therapeutic Target for Human Follicular and Anaplastic Thyroid Cancer

doi: 10.1016/j.omto.2020.01.003

Figure Lengend Snippet: Effect of siRNA Gene Silencing on p53, p27, and cyclin D 1 Expression Levels through Western Blotting Analysis on FTC-133 and 8305C Cell Line Cultures (A) Representative immunoblots of p53, p27, and cyclin D 1 expression levels in total cellular lysates from scrambled FTC-133 and 8305C human thyroid cancer cell lines and 10 nM siRNA-TfR1-transfected cells for 48 h. (B) Densitometric analysis of p53, cyclin D 1 , and p27 (performed after normalization with β-tubulin) in response to siRNA-TfR1 transfection, when compared with scrambled ones, used as control. Results are expressed as the mean ± SD of the values of five separate experiments performed in triplicate. *p < 0.05 for differences versus controls.

Article Snippet: Follicular (FTC-133) and anaplastic (8305C) human thyroid cancer cells lines were obtained from the Cell Bank Interlab Cell Line Collection (Genoa, Italy).

Techniques: Expressing, Western Blot, Transfection, Control

Journal: Molecular Therapy Oncolytics

Article Title: Gene Silencing of Transferrin-1 Receptor as a Potential Therapeutic Target for Human Follicular and Anaplastic Thyroid Cancer

doi: 10.1016/j.omto.2020.01.003

Figure Lengend Snippet: Effect of siRNA Gene Silencing on Caspase-3 Cleavage through Western Blotting Analysis on FTC-133 and 8305C Cell Line Cultures (A) Representative immunoblot of caspase-3 cleavage in total cellular lysates from scrambled FTC-133 and 8305C human thyroid cancer cell lines and 10 nM siRNA-TfR1 transfected cells for 48 h. (B) Densitometric analysis of caspase-3 (performed after normalization with β-tubulin) in response to siRNA-TfR1 transfection, when compared with scrambled ones, used as control. Results are expressed as the mean ± SD of the values of five separate experiments performed in triplicate. *p < 0.05 for differences versus controls.

Article Snippet: Follicular (FTC-133) and anaplastic (8305C) human thyroid cancer cells lines were obtained from the Cell Bank Interlab Cell Line Collection (Genoa, Italy).

Techniques: Western Blot, Transfection, Control

Journal: Molecular Therapy Oncolytics

Article Title: Gene Silencing of Transferrin-1 Receptor as a Potential Therapeutic Target for Human Follicular and Anaplastic Thyroid Cancer

doi: 10.1016/j.omto.2020.01.003

Figure Lengend Snippet: Effect of siRNA Gene Silencing on DNA Fragmentation through TUNEL Test on FTC-133 and 8305C Cell Line Cultures (A and B) Representative images of TUNEL assay performed in (A) FTC-133 and (B) 8305C human thyroid cancer cell lines scrambled (a and c) and 10 nM siRNA-TfR1-transfected cells for 48 h (b and d). Immunostaining of non-apoptotic (red) and apoptotic (green) cells is shown. Magnification is equal in all images. Scale bars, 20 μm.

Article Snippet: Follicular (FTC-133) and anaplastic (8305C) human thyroid cancer cells lines were obtained from the Cell Bank Interlab Cell Line Collection (Genoa, Italy).

Techniques: TUNEL Assay, Transfection, Immunostaining